The objective of this study was to determine the effect of nitrogen and phosphorus on the growth and total chlorophyll content of a freshwater microalgae Scenedesmus sp. Growth behavior under modified Chu-10 medium without nitrogen source showed strong inhibition with growth rate reduced to 72.84% after 96 hour of treatment. There was about 10.24% inhibition of growth when the algae were cultured in a modified Chu-10 medium without phosphorus source. The photosynthetic pigment such as total chlorophyll content decreased at the same extent after 96 hour of culture in modified Chu-10 medium without the nitrogen source but not decreases in the medium without phosphorus source and the inhibition was highest in the medium without the nitrogen source.