Evaluation of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage in mosquito larvae treated with plant extracts

Lalrotluanga, N. Senthil Kumar, G. Gurusubramanian

Science Vision 11 (3), 155-158 | Published on 30 September 2011 |

Abstract

The random amplified polymorphic DNA (RAPD) assay was used to assess the level of DNA damage in various exposed and unexposed Culex quinquefasciatus larvae to acetone and chloroform extracts of Curcuma longa and Melia azedarach at different concentrations (6.25, 12.5 and 25 ppm). This is the first report of an analysis of genomic alterations in plant extracts-treated mosquito larvae using RAPD-PCR fingerprinting. In comparison to the control larvae, larvae treated with the plant extracts caused greater changes in the RAPD patterns. DNA strand breakage was more in the larvae of C. quinquefasciatus.

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