Volume 23, Issue 4, 2023 October - December

Volume 23, No 4 Pages:
2023 October - December Articles: 3

Rearing Protocol for Culex quinquefasciatus

Mosquitoes are vectors capable of transmitting various life-threatening diseases such as dengue, chikungunya, Japanese encephalitis, lymphatic filariasis, malaria, and so on. They are reared in the laboratories for conducting various studies such as vector biology, vector competence, mechanism of disease transmission, to check the efficiency and follow-up of various control methods, insecticide susceptibility, and vaccine trials. Moreover, mass rearing of mosquitoes is required for sterile insect technique (SIT) to control mosquitoes. Successful breeding of mosquitoes requires attention to detail and depends upon various factors such as quality and quantity of larval food, temperature, humidity, population size, mating, blood feeding, and egg laying. Culex quinquefasciatus is a vector of lymphatic filariasis (LF) and a cosmopolitan mosquito, abundant in tropical and subtropical regions. In this study, a simple and convenient rearing protocol for Culex quinquefasciatus has been discussed.

Depositional Environment and Provenance of Tipam Sandstone Formation Exposed in Parts of Naga Schuppen Belt, Naga Hills, NE India

Naga Hills, one of the seven sisters of NE India provides a unique opportunity to study Tertiary Geology. Three different morphotectonic belts of this geological terrain, i.e. Inner Fold Belt, Belt of Schuppen and the Naga Ophiolite Belts are home to the sediments of different times between Late Cretaceous to Recent. The focus of this study is situated in the Belt of Schuppen, the trust belt bounded by Naga thrust and Disang thrust. The age of the sediments of this belt is ranged from Oligocene to Recent. An economically important sedimentary unit the Tipam Group of rocks is well exposed in this belt. In this study, an approach has been made to understand the depositional environment and provenance characteristics of the Tipam Sandstone Formation of Tipam Group exposed in and around Kukidolong in Dimapur district. By the study of different facies parameters, the rocks of the study area have been divided into two lithofacies namely: Planar crossbedded medium-grained sandstone (MSp) and Trough cross-bedded mediumgrained sandstone facies (MSt). The facies characteristics and sedimentary structures observed in the area point towards a mid-channel- bar depositional system of a river as mighty as Brahmaputra. The heavy mineral assemblages found in this sandstone interpret a mixed provenance of igneous, metamorphic and sedimentary rocks

Mycobiota Associated with Dacryodes edulis H. J. Lam fruits sold In Rumokoro Market, Port Harcourt, Nigeria

Dacryodes edulis H. J. Lam is a common fruit in Nigeria used to eat roasted or boiled corn. It can also be eaten alone by softening with hot water, hot ash, or by roasting on low heat. It is called “ube” in the eastern part of Nigeria. D. edulis fruits are prone to infection by fungal pathogens in the field, in transit and during storage. Proper identification of these pathogens is important in disease prevention and control. This study was carried out to isolate and identify the fungal species associated with D. edulis fruits. Fruits were obtained from Rumokoro market, Rivers State, Nigeria in January 2020. Isolation of fungi was done by Potato Dextrose (PDA) method and identification was carried out by molecular method. Fungal DNA was extracted using the Zymo Fungal/Bacterial DNA Miniprep Kit. Polymerase chain reaction amplification of the internal transcribed spacer (ITS) region was carried out using the primer pair: ITS4 and ITS5. BLAST search was carried out on the National Centre for Biotechnology Information (NCBI) database and the fungi were identified as: Rhizopus delemar, Aspergillus oryzae and A. welwitschiae. The Phylogenetic tree was constructed to show the evolutionary relationship among the isolates and other fungal species retrieved from GenBank. The sequences of the isolates have been deposited in GenBank under the accession numbers: ON965489, ON965490 and ON965491 for Rhizopus delemar, Aspergillus oryzae and A. welwitschiae respectively. The molecular method used in this study enabled the isolates to be identified at the species levels.